Eosinophil extracellular trap cell death–derived DNA traps: Their presence in secretions and functional attributes

Functional extracellular eosinophil granules: a bomb caught in a trap.

Eosinophils store a wide range of preformed proteins, including cationic proteins and cytokines, within their morphologically unique granules. Recently, we have demonstrated that cell-free eosinophil granules are functional, independent, secretory organelles and that clusters of cell-free granules are commonly found at tissue sites associated with various pathologic conditions. Cytolytic releas...

Eosinophil extracellular DNA trap cell death mediates lytic release of free secretion-competent eosinophil granules in humans.

Eosinophils release their granule proteins extracellularly through exocytosis, piecemeal degranulation, or cytolytic degranulation. Findings in diverse human eosinophilic diseases of intact extracellular eosinophil granules, either free or clustered, indicate that eosinophil cytolysis occurs in vivo, but the mechanisms and consequences of lytic eosinophil degranulation are poorly understood. We...

Extracellular DNA traps promote thrombosis.

Neutrophil extracellular traps (NETs) are part of the innate immune response to infections. NETs are a meshwork of DNA fibers comprising histones and antimicrobial proteins. Microbes are immobilized in NETs and encounter a locally high and lethal concentration of effector proteins. Recent studies show that NETs are formed inside the vasculature in infections and noninfectious diseases. Here we ...

Highlight : Eosinophil extra - cellular traps in Allergy

Eosinophil degranulation has always aroused the interests of physicians and scientists alike. Loss of viability and release of membrane-free granules upon eosinophil degranulation was first demonstrated by Gleich and coworkers in 1996. These free eosinophil granules, once considered as crush artifacts, are now confirmed products of eosinophil cytolysis, and suggested to be significantly pathoge...

Visualization and Functional Evaluation of Phagocyte Extracellular Traps